Figure 5
From: Investigation of Tumor Cell Behaviors on a Vascular Microenvironment-Mimicking Microfluidic Chip
Tumor cells-ECs monolayer interaction in TNF-α conditioned biochemical environment.
(a) The morphological change of the ECs monolayer in the absence or in the presence of TNF-α. (b) The adhesion of the tumor cells on the ECs monolayer and their morphological changes in the absence of TNF-α. (c) The adhesion of the tumor cells on the ECs monolayer and their morphological changes in the presence of TNF-α. In (b,c), the tumor cells were stained with Calcein Green AM. Scale bar, 20 μm. (d) The number of adhered tumor cells on the ECs monolayer in the absence or presence of TNF-α. (e) The spreading area of adhered tumor cells on the ECs monolayer in the absence or presence of TNF-α. (f) The aspect ratio of the adhered tumor cells in the absence or presence of TNF-α. (g) The result of flow cytometry indicates the apoptosis rate of the tumor cells in the absence of TNF-α at static condition for 24 h. (h) The result of flow cytometry indicates the apoptosis rate of the tumor cells in the presence of TNF-α at static condition for 24 h. (i) Statistical data of the tumor cell apoptosis in the absence or presence of TNF-α at static condition for 24 h. *Conditions that are statistically different (P < 0.05). **Conditions that were statistically different (P < 0.01). All results are shown plotted as mean ± SD with each group containing three experiments. Scale bar, 20 μm.
