Figure 1

Strip interacts with microtubule.

(a) Drosophila S2 cells plated on a concanavalin-A coated cover slip were immunostained with anti-Strip (green) and anti-α-tubulin (magenta) antibodies. Scale bar is 7.5 μm. (b) S2 cells were treated with the extraction method for clear observation of microtubules. S2 cells are immunostained with anti-Strip (green) and anti-α-tubulin (magenta) antibodies. Dotted rectangle area is enlarged in the right panels. Scale bar is 7.5 μm. (c) S2 cell extract was incubated with in vitro-polymerized microtubules or buffer only and pelleted by centrifugation. The pellet was denatured and subjected to western blotting using anti-Strip and anti-α-tubulin antibodies.