Figure 4
From: A STRIPAK component Strip regulates neuronal morphogenesis by affecting microtubule stability
Strip cooperates with Dscam in the morphogenesis of mushroom body neurons.
(a,b) Representative images of control (a) or stripdogi (b, 11.8%, n = 8/68) homozygous clones of mushroom body α/β neurons. UAS-mCD8-GFP (green) is expressed by OK107-Gal4. Anti-FasII antibody was used to visualize MB α/β lobes (magenta). Scale bar is 50 μm. (c–e) Representative images of mushroom body neurons for each genotype. UAS-mCD8-GFP (green) is expressed by OK107-Gal4. Anti-FasII antibody was used to visualize MB α/β lobes (magenta). Scale bar is 50 μm. (f) Quantification of phenotypes of α and β lobes. **P = 0.0075, ***P < 0.0001, chi-square test. (g,h) Immunoprecipitation of S2 lysates expressing Dscam17.1-GFP. Dscam17.1-GFP was co-immunoprecipitated when Strip was precipitated by anti-Strip antibody (g). Endogenous Strip was co-immunoprecipitated when Dscam17.1-GFP was precipitated by anti-GFP antibody (h). Genotypes: (a) y w, hs-flp, UAS-mCD8GFP/y w (or Y); UAS-βtub56D-myc/+; tub-Gal80, FRT2A/FRT2A, y(+); OK107-Gal4/+ (b) y w, hs-flp, UAS-mCD8GFP/y w (or Y); UAS-βtub56D-myc/+; tub-Gal80, FRT2A/stripdogi, FRT2A, y(+); OK107-Gal4/+ (c) y w, hs-FLP, UAS-mCD8GFP/w (or Y); +/Cy, y+; OK107-Gal4/+, (d) y w, hs-FLP, UAS-mCD8GFP/y w (or Y); Dscam17.2GFP/Cy, y+;; OK107-Gal4/+, (e) y w, hs-FLP, UAS-mCD8GFP/y w (or Y);; stripdogi, FRT2A, y+/TM3, Sb; OK107-Gal4/+.
