Figure 4 | Scientific Reports

Figure 4

From: A STRIPAK component Strip regulates neuronal morphogenesis by affecting microtubule stability

Figure 4

Strip cooperates with Dscam in the morphogenesis of mushroom body neurons.

(a,b) Representative images of control (a) or stripdogi (b, 11.8%, n = 8/68) homozygous clones of mushroom body α/β neurons. UAS-mCD8-GFP (green) is expressed by OK107-Gal4. Anti-FasII antibody was used to visualize MB α/β lobes (magenta). Scale bar is 50 μm. (ce) Representative images of mushroom body neurons for each genotype. UAS-mCD8-GFP (green) is expressed by OK107-Gal4. Anti-FasII antibody was used to visualize MB α/β lobes (magenta). Scale bar is 50 μm. (f) Quantification of phenotypes of α and β lobes. **P = 0.0075, ***P < 0.0001, chi-square test. (g,h) Immunoprecipitation of S2 lysates expressing Dscam17.1-GFP. Dscam17.1-GFP was co-immunoprecipitated when Strip was precipitated by anti-Strip antibody (g). Endogenous Strip was co-immunoprecipitated when Dscam17.1-GFP was precipitated by anti-GFP antibody (h). Genotypes: (a) y w, hs-flp, UAS-mCD8GFP/y w (or Y); UAS-βtub56D-myc/+; tub-Gal80, FRT2A/FRT2A, y(+); OK107-Gal4/+ (b) y w, hs-flp, UAS-mCD8GFP/y w (or Y); UAS-βtub56D-myc/+; tub-Gal80, FRT2A/stripdogi, FRT2A, y(+); OK107-Gal4/+ (c) y w, hs-FLP, UAS-mCD8GFP/w (or Y); +/Cy, y+; OK107-Gal4/+, (d) y w, hs-FLP, UAS-mCD8GFP/y w (or Y); Dscam17.2GFP/Cy, y+;; OK107-Gal4/+, (e) y w, hs-FLP, UAS-mCD8GFP/y w (or Y);; stripdogi, FRT2A, y+/TM3, Sb; OK107-Gal4/+.

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