Figure 7

Copy number estimation of SRY, BPY2 and DAZ genes using real time PCR.
(aād) Summarizes the distribution of copy number of SRY, BPY2 and DAZ in oligospermic (a), Azoospermic (b), infertile males with normal spermiogram (c) and normal fertile males (d), respectively. X-axis depicts the gene of interest analyzed and the copy number assessed using real time PCR while the Y-axis indicates the % of males with corresponding copies for the genes. Grey bars indicate the normal copy number for the particular gene and the white bars indicate the copy number variation observed. A total of 43 OS, 34 AZ and 40 INS patients were analyzed for copy numbers and each reaction was set in triplicates during real time PCR amplification.