Table 2 Effect of storage method (−20 °C versus 95% ethanol) on the geochemical compositions of ontogenetic shell stages (prodissoconch and dissoconch) based on PERMANOVA.

From: Effects of sample storage and shell orientation on LA-ICPMS trace element measurements on deep-sea mussels

  

All Elements

Mg:Ca

Sr:Ca

Pb:Ca

U:Ca

Ba:Ca

Source

df

MS

Pseudo-F

MS

Pseudo-F

MS

Pseudo-F

MS

Pseudo-F

MS

Pseudo-F

MS

Pseudo-F

 Shell stage

1

125.2

66.05****

28.73

104.3****

32.49

196.0****

4.086

4.253*

30.98

141.8****

28.93

105.1****

 Stor. method

1

0.333

0.175ns

0.070

0.255ns

0.068

0.413ns

0.002

0.002ns

0.073

0.332ns

0.12

0.434ns

 Stage x Stor.

1

1.2

0.633ns

0.28

1.016ns

0.475

2.868ns

0.327

0.340ns

0.084

0.383ns

0.035

0.127ns

 Residuals

36

1.896

 

0.275

 

0.166

 

0.961

 

0.218

 

0.275

 

Prodissoconch

 Stor. method

1

3.606

0.710ns

0.907

0.902ns

1.872

1.967ns

0.150

0.143ns

0.374

0.362ns

0.304

0.292ns

 Residuals

18

5.077

 

1.005

 

0.952

 

1.047

 

1.035

 

1.038

 

Dissoconch

 Stor. method

1

1.536

0.296ns

0.180

0.172ns

0.906

0.901ns

0.209

0.200ns

0.038

0.036ns

0.202

0.194ns

 Residuals

18

5.192

 

1.046

 

1.005

 

1.044

 

1.053

 

1.044

 
  1. df = degrees of freedom; MS = mean sum of squares; Pseudo-F = F value by permutation;
  2. ****P(perm) < 0.0001; *P(perm) < 0.05; ns: not significant.
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