Figure 3

[³H]XA binding in membranes prepared from transfected HEK293 cells or mouse cortical membranes.

(A,B) Binding of [³H]XA (5 nM) in membranes prepared from HEK293 cells expressing mGlu2 or mGlu3 receptors. Values are means + S.D. of 2–3 determinations. *p < 0.05 (One-way ANOVA + Fisher’s LSD; F values: (A) F_(6,19) = 5.73; (B) F_(6,20) = 23.24. (C) Binding of [³H]XA binding (5 nM) in membrane prepared from HEK293 cells expressing mGlu2 receptors incubated in the presence of orthosteric or allosteric mGlu2 receptor ligands. Values are means + S.D. of 3–6 determinations. *p < 0.05 (One-way ANOVA + Fisher’s LSD; F_(7,32) = 2.56). (D) Excessive concentrations of XA (1 mM) fail to inhibit [³H]XA binding in membranes from HEK293 cells expressing mGlu4 receptors, or in membranes from mock cells. Values are means + S.D. of 3–6 determinations. (E) Concentration-dependent inhibition of [³H]XA (5 nM) binding by non-radioactive XA in mouse cortical membranes. The lack of effect of mGlu and NMDA receptor ligands on [³H]XA binding is shown in the inset. (F) [³H]LY341495 (1 nM) binding in mouse cortical membranes incubated in the presence of increasing concentrations of XA or LY379268. In (E,F), values are means ± S.D. of triplicates.