Table 1 Composition of electroporation buffers.
From: Electroporation on microchips: the harmful effects of pH changes and scaling down
KCl | KH2PO4 | K2HPO4 | myo-Inositol | pH value | Osmolarity (mOsmol/kg) | |
|---|---|---|---|---|---|---|
Solution without phosphate saline | 27 mM | 0 | 0 | 36 mM | 7.0 | ~90 |
Eppendorf hypoosmolar electroporation buffer* | 25 mM | 0.3 mM | 0.85 mM | 36 mM | 7.4 | ~90 |
Modified electroporation buffer | 0 | 13 mM | 13 mM | 25 mM | 7.2 | ~90 |
