Figure 3
Transcriptional gene silencing is suppressed in the hda6–9 and hda6–10 mutant plants.
(A) Expression levels of LUC (upper panel), endogenous RD29A (middle panel) and NPTII (lower panel) relative to those in ros1–1 were measured with qRT-PCR using UBQ3 as an internal reference. The Y-axis represents the fold changes compared to ros1–1. Ten-day-old seedlings were treated at 4 °C for 1 day and then total RNAs were extracted for analyzing the expression of the reporter genes and endogenous genes. (B) RNA blot assays showed the expression level of NPTII in wild type, ros1–1, hda6–9, hda6–10 and ros1 ago4. UBQ3 was used as a loading control. (C) Expression of selected endogenous targets of HDA6 determined by RT-PCR with random hexamer primers. ACT2 (ACTIN2) (its CT mean in all 4 samples with 20 cycles) was used as an internal reference. (D) Relative expression of Group E target ERT7, 35S::NPTII and SDC among the two hda6 mutated alleles and ros1 hda6–6 (the hda6 null mutant axe1–5 in the ros1–1 background). Data are means and standard derivations based on 3 biological replicates.
