Figure 2
From: Quantitating intracellular oxygen tension in vivo by phosphorescence lifetime measurement
BTPDM1 characteristics in HK-2 cells.
(a) Phosphorescence/fluorescence image of BTPDM1, LysoTracker, merged image and bright field image of HK-2 cells. The brightness and contrast were adjusted from original in a bright field image. Scale bar: 50 μm. (b) Phosphorescence lifetime of BTPDM1 in HK-2 cells in various pO2 of incubating atmosphere at 37 °C. Each phosphorescence lifetime is the average of four measurements. Error bar: S.D. (c) Reciprocal plot of phosphorescence lifetime and oxygen concentration. The approximate line was made by a straight-line approximation and an approximation formula and the coefficient of determination were also shown.
