Figure 4
From: Quantitating intracellular oxygen tension in vivo by phosphorescence lifetime measurement
Phosphorescence lifetime change in hypoxic conditions of kidney.
Experimental schemes (left panels) and phosphorescence lifetime alteration (right panels). The values written under graphs are converted pO2 (mmHg) from phosphorescence lifetimes by using the calibration line. (a) Phosphorescence lifetime alteration in acute ischemia/reperfusion. Acute ischemia and reperfusion were induced by clamping and declamping left renal artery and vein; n = 3. (b) Phosphorescence lifetime alterations in changing inspired oxygen concentration. The oxygen concentration of inhaling atmosphere was changed by digital gas mixing system; n = 4 (changing group), n = 3 (control group). (c) Phosphorescence lifetimes in acute anemia model. Anemia was induced by phlebotomy through the tail vein; n = 4 (control group), 6 (anemia group). In these three experiments, phosphorescence lifetimes actually elongated in hypoxic kidneys. Error bar: S.D. #P < 0.05 by two-tailed unpaired t-test.
