Table 1 Strains and plasmids for alterations.

From: Seven gene deletions in seven days: Fast generation of Escherichia coli strains tolerant to acetate and osmotic stress

Strain

Genotype

Reference

Strains

 Escherichia coli K-12 MG1655

F- λ- ilvG- rfb-50 rph-1

A. Feist1

 Escherichia coli W

F-, soil isolate, harbors pRK1 and pRK2

DSMZ 1116

 SIJ488

E. coli K-12 MG1655Tn7::para-exo-beta-gam; prha-FLP; xylSpm-IsceI

This study

Plasmid

Description

Reference

Plasmids for deletions

 pSIJ8

pkd46, rhaRS-prha-FLP, amp

This study

 pSIJ214

pEMG, MG1655-tn7-para-exo-beta-gam; prha-flp; xylSpm-I-SceI, km

This study

Plasmids for frt flanked deletion cassettes

 pkd3

ApR, FRT-cmR-FRT, oriR6K

37

 pkd4

ApR, FRT-KmR-FRT, oriR6K

37

 pSIJ196

pEMG amp, frt::spec::frt

This study

 pSIJ197

pEMG amp, frt::gm::frt

This study

  1. 1This strain was generously donated by Dr. Adam Feist, University of California, San Diego.
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