Table 2 Quantitative real-time RT-PCR (qRT-PCR) results of five selected genes in oocytes, 4-cell and blastocyst stage embryos.

From: mRNA Levels of Imprinted Genes in Bovine In Vivo Oocytes, Embryos and Cross Species Comparisons with Humans, Mice and Pigs

Gene symbol

Oocyte vs. 4-cell

Oocyte vs. Blastocyst

Log (Fold change) RNA-seq

Log (Fold change)*qRT-PCR

Log (Fold change) RNA-seq

Log (Fold change)*qRT-PCR

CDKN1C

3.0

3.6

1.8

2.5

MEST

1.9

2.5

9.7

9.8

PLAGL1

2.0

2.3

12.3

12.4

PHLDA2

−2.5

−2.8

−0.1

−1.4

GNAS

−0.6

−1.5

−4.7

−5.1

  1. *Fold change is expressed as the ratios of the values of the oocyte (n = 3) divided by those of the 4-cell and blastocyst embryos (n = 3), respectively. Real time RT-PCR results substantiated the differential gene expression patterns from RNA-seq.
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