Figure 1

Human intestinal cells promote growth of F. prausnitzii in the HoxBan system.
(a) Schematic drawing of the HoxBan coculture system with F. prausnitzii growing in solid YCFAG agar overlaid with liquid DMEM medium and Caco-2 cells on coverslips facing the agar. (b) Pictures documenting F. prausnitzii colony formation in the absence and presence of Caco-2 cells after 18 h (top panel) and 36 h (bottom panel) in air-open and air-tight culture tubes. (c) F. prausnitzii colony formation after 18 h coculture with Caco-2 (left), DLD-1 (middle) and HepG2 cells (right).