Figure 1

Establishment of a CD161-based immunological measure to diagnose active tuberculosis.

(A) Gating strategy to enumerate CD161⁺ populations and the proposed formulas to compare active tuberculosis and LTBI or healthy control. Lymphocytes (CD45⁺CD64⁻) and monocytes (CD45⁺CD64⁺) were gated by the CD45 marker and then separated by the presence and absence of CD64. The different subsets of lymphocytes were gated via different combinations of CD3, CD4 and CD8 markers. Note CD4⁺CD161⁺ and CD8⁺CD161⁺ cells are also CD3⁺. (B) Gating and enumeration of CD161⁺ populations from specimens of 9 participants, consistently showing clear separation of cell groups. (C) Comparison between the LTBI or HC cohort and the TB cohort using the percentages of CD3⁺CD161⁺, CD4⁺CD161⁺ and CD8⁺CD161⁺ cells. *P < 0.05.