Figure 2
From: The Dynamics and Regulatory Mechanism of Pronuclear H3k9me2 Asymmetry in Mouse Zygotes
The influence of BIX 01294 and G9a or GLP antibody microinjection on H3K9me2.
(A) The H3K9me2 state of zygotes at 12 h of IVF treated with BIX 01294. CHX and BIX indicate zygotes cultured in KSOM medium containing cycloheximide and BIX01294, respectively. BIX+CHX indicates zygotes cultured in KSOM medium containing cycloheximide and BIX01294 together. (B) The H3K9me2 state of zygotes at 12 h of IVF after G9a or GLP antibody microinjection. α -G9a and α –GLP indicates zygotes cultured in normal KSOM medium after antibody microinjection. And α -G9a+CHX and α –GLP+CHX indicates zygotes cultured in KSOM medium containing cycloheximide after antibody microinjection. White arrow indicates the increased H3K9me2 of the male pronucleus and red arrows indicate the low H3K9me2 level of the male pronucleus. Scale bar = 20 μm.
