Figure 3
From: The Dynamics and Regulatory Mechanism of Pronuclear H3k9me2 Asymmetry in Mouse Zygotes
The influence of G9a or GLP siRNA microinjection on H3K9me2.
(A) The mRNA and protein expression of G9a were detected after 24 h of G9a siRNA microinjection (G9a-si). L, the G9a long isoform. S, the G9a short isoform. *P < 0.01. (B) The mRNA and protein expression of GLP were detected after 24 h of GLP siRNA microinjection (GLP-si). Actb (beta-actin) was used as a loading control. *P < 0.01. (C) The H3K9me2 state of zygotes at 12 h of IVF after G9a or GLP siRNA microinjection. NC (negtive control) indicates zygotes microinjected with scrambled control siRNA. NC+CHX, G9a-si+CHX and GLP-si+CHX indicate zygotes cultured in KSOM medium containing cycloheximide after siRNA microinjection. White arrow indicates the increased H3K9me2 of the male pronucleus and red arrow indicates the low H3K9me2 level of the male pronucleus. Scale bar = 20 μm.
