Figure 3 | Scientific Reports

Figure 3

From: A novel, native-format bispecific antibody triggering T-cell killing of B-cells is robustly active in mouse tumor models and cynomolgus monkeys

Figure 3

Redirected T cell-mediated cytotoxicity of bispecific antibodies in vitro.

(a,b) Effects of treatment of CD20 positive Raji cells with CD20 x CD3 bispecific antibodies and pre-activated human (a) or cynomolgus (b) effector lymphocytes (E:T-ratio 10:1). Cell viability was measured by Calcein release assay after 2 hours of incubation. (c) Specific lysis of CD20+ target cells upon treatment with 10 ug/ml of CD20 x CD3 bispecific antibodies and effector lymphocytes (E:T ratio 4:1). B16F10.9 cells expressing exogenous CD20 are labeled with Violet cell tracker (upper left quadrants), while control B16F10.9 cells are labeled with CFDA-SE (lower right quadrants). Both REGN2280 and REGN1979 selectively deplete cells expressing CD20. (d) Effects of treatment of CD20-positive B16F10.9 cells with CD20 x CD3 bispecific antibodies and effector lymphocytes (E:T ratio 4:1). Cell viability was measured by FACS after 48 hours incubation. (e) Dose-dependent effect of the CD20 x CD3 bispecific antibodies on the expression of CD69 on CD2+ lymphocytes in the presence of B16F10.9 expressing CD20 cells after incubation for 48 hours. The surface expression of CD69 on effector cells was detected by flow cytometry using an antigen-specific PE conjugated antibody.

Back to article page