Figure 5
From: PDGFRα plays a crucial role in connective tissue remodeling
Fibronectin deposition is impaired in implanted sponge in α-KO mice.
(a) Immunohistochemical analysis of the fibronectin deposition (brown) in connective tissue-formed sponges. Representative images of male Flox (left) and α-KO (right) mice at day 14 after implantation. α-KO mice show lesser extent of immunoreactivity to fibronectin compared to Flox mice. (b) In α-KO mice, the fibronectin-depositing area is significantly less than in Flox mice. ***p < 0.001 versus Flox mice. n = 4 mice per group. (c) In Flox mice, integrin α5 (red), a component of fibronectin receptor dimer, is expressed in CD31-positive blood vessels (green) within the sponge (upper row). Similarly, integrin α5 is expressed in CD31-positive blood vessels of ingrowing connective tissue in the α-KO mice (bottom row). Nuclei are depicted by Hoechst (blue) and sponges are visible due to the autofluorescence (cyan). Scale bars indicate 100 μm (a) and 50 μm (c).
