Figure 4
Dengue virus purified from infected human bone marrow demonstrated the same characteristics as virus derived from acute patient plasma.
(a) DENV from infected human bone marrow were concentrated as described in the Methods and subjected to electron microscopy (EM), cryoEM (b) and immunoEM (c) confirming the virus particles to be indeed dengue virus. (d) Further investigations showed that the dengue vesicles from human bone marrow could also regain the DENV capsid content when amplified in Vero cells, showing the dual life cycle property as seen with DENV from patient serum. (e) Protein profiling of virus derived from infected human bone marrow was assayed with Western blot. The results were similar to that of DENV isolated from acute serum, which did not contain capsid but expressed comparable levels of other structure proteins and NS1 levels.
