Table 1 Properties of red FPs with emission maxima above 600 nm.
From: Monomeric Garnet, a far-red fluorescent protein for live-cell STED imaging
Protein | Ex/Em (nm) | QY | EC (M−1cm−1) | MB relative to mRuby (%) | pKa | Maturation half-time at 37 °C (min) | Fluorescence Lifetime (ns) |
|---|---|---|---|---|---|---|---|
Monomers | |||||||
mRuby | 558/605 | (0.35)19 | (112,000)19 | 100 | (4.4)19 | (168)19 | (2.6)19 |
mRaspberry | 598/625 | (0.15)25 | (79,000)25 | 30 | (5.0)17 | (126)17 | ND |
mKate2 | 588/633 | (0.40)38 | (62,500)38 | 64 | (6.5)17 | (48)17 | ND |
mKate | 588/635 | (0.28)38 | (31,500)38 | 23 | (6.5)25 | (75)25 | ND |
mPlum | 590/649 | (0.10)25 | (22,000)25 | 6 | (5.5)17 | (96)17 | ND |
mNeptune1 | 600/650 | (0.20)26 | (67,000)26 | 34 | (5.4)26 | (28)27 | ND |
mNeptune2 | 599/651 | (0.24)27 | (89,000)27 | 54 | (6.3)27 | (27)27 | ND |
mNeptune2.5 | 599/643 | (0.28)27 | (95,000)27 | 68 | (5.8)27 | (26)27 | ND |
mCardinal | 604/659 | (0.19)27 | (87,000)27 | 42 | (5.3)27 | (27)27 | ND |
TagRFP657 | 611/657 | (0.10)18 | (34,000)18 | 9 | (5.0)18 | (125)18 | 1.4 |
mGarnet | 598/670 | 0.09 | 95,000 | 22 | 7.4 | 112 | 0.8 |
Dimers | |||||||
eqFP650 | 592/650 | (0.24)22 | (65,000)22 | 40 | (5.7)22 | ND | ND |
eqFP670 | 605/670 | (0.06)22 | (70,000)22 | 11 | (4.5)22 | ND | ND |
Tetramers | |||||||
eqFP611 | 559/611 | (0.45)20 | (78,000)20 | 90 | ND | ND | (2.5)20 |
RFP639 | 588/639 | (0.18)35 | (69,000)35 | 32 | (<4.0)35 | (90)35 | ND |
E2-Crimson | 605/646 | (0.12)17 | (58,500)17 | 18 | (4.5)17 | (26)17 | ND |
