Figure 1
Schematic illustration of the experiment and representative results.
(a) Schematic illustration of the cell-free protein synthesis system. Membrane proteins were individually expressed with a reconstituted cell-free translation system, the PURE system, in the absence or presence of liposomes. Each translation product was labeled with [35S]-methionine. After translation, the uncentrifuged total fraction (Total) and the supernatant fraction after centrifugation (Sup) were subjected to SDS-PAGE and quantified by autoradiography. The ratio of the translation products in the Total and Sup fractions was defined as the solubility, which represents the protein’s aggregation propensity. The data obtained in this experiment were analyzed statistically. (b) SDS-PAGE of four E. coli membrane proteins (YfbF, CyoE, ZnuB and DinF) synthesized in the absence or presence of liposomes. (c) Liposome concentration-dependence of the solubility of four translated membrane proteins.
