Figure 8 | Scientific Reports

Figure 8

From: Dioscin alleviates alcoholic liver fibrosis by attenuating hepatic stellate cell activation via the TLR4/MyD88/NF-κB signaling pathway

Figure 8

Both MyD88 and NF-κB are involved in inhibition of HSCs activation by dioscin.

(A,B) Effects of dioscin on the levels of TLR4, MyD88, NF-κB, TGF-β1, p-Smad2/ Smad2, α-SMA and COL1A1 with or without ST2825 and PDTC in HSC-T6 and LX2 cells. The cropped gels are used and full-length gels are presented in Supplementary Fig. S11. (C,D) Effects of dioscin on the mRNA levels of IL-1, IL-6 and TNF-α measured by quantitative real-time PCR assay in HSC-T6 and LX2 cells. (E,F) Effects of dioscin on the level of secreted COL1A1 in supernatants measured by ELISA in HSC-T6 and LX2 cells. The values are expressed as the mean ± SD (n = 3). *p < 0.05 and**p < 0.01 vs. model group; and &p < 0.05 and &&p < 0.01 vs. LPS + Dio + ST2825 group.

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