Figure 6
From: Analysis of histone modifications at human ribosomal DNA in liver cancer cell
Effect of UBF depletion on the distribution of histone modification marks (H3K4me3, H3K36me3, H3K27me3 and H3K4me1) at rDNA in human liver cancer cell.
(A) Loss of UBF leads to a decrease of H3K4me3 at H42 region of rDNA. ChIP-QPCR analysis of different regions of rDNA in HepG2 cells transfected with control or UBF siRNA using antibodies against H3K4me3. The ratio of rDNA occupancy between UBF knockdown cells and control cells was calculated for each antibody. Student’s t-test was performed between cells transfected with or without UBF siRNA. (B–C) Depletion of UBF does not alter H3K36me3 and H3K4me1 distribution at rDNA. ChIP-QPCR analysis of different regions of rDNA in HepG2 cells transfected with control or UBF siRNA using antibodies against H3K36me3 (B), H3K4me1 (C). The ratio of rDNA occupancy between UBF knockdown cells and control cells was calculated for each antibody. Student’s t-test was performed between cells transfected with or without UBF siRNA.
