Figure 5 | Scientific Reports

Figure 5

From: Discovery of novel INK4C small-molecule inhibitors to promote human and murine hematopoietic stem cell ex vivo expansion

Figure 5

p18SMI compounds increase HSC proliferation through the p18-CDK4/6 pathway in LT-HSC.

(A) CFSE analysis of bone marrow cells cultured with cytokine plus p18SMI compound to assess the number of cell divisions. Top percentage, first generation; second percentage, second generation; third percentage, third generation; and bottom percentage, fourth generation. (B) Specific dependence of compound 40 on CDK4/6, rather than CDK2. (C) A model of cell-cycle regulation by p18 and CDKs. p18SMI-induced HSC expansion acts through p18-CDK4/6 pathway in LT-HSC. Data is mean ± SEM for all experiments of two or more and were performed in duplicate or triplicate.

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