Figure 6
Cytotoxicity assessment of top p18SMI compounds.
(A) Cell proliferation assay was performed on 32D cells to evaluate general cytotoxicity. The cell survival was determined by the MTT assay. (B) Apoptosis statistics analysis of cultured c-Kit enriched BM cells which were cultured with cytokine plus 20 μM compound 40 or DMSO. Uncultured bone marrow cells were irradiated by UV to serve as positive control. Apoptosis was checked by Annexin V staining on hematopoietic cells. (C,D) Proliferation effect of compound 40 on leukemia cell lines KG-1 and KG-1a. The cell survival was determined with the MTT assay. Data is mean ± SEM for all experiments of two or more and were performed in duplicate or triplicate.
