Figure 2

Induction of LdNAT1 expression by juvenile hormone (JH) in Leptinotarsa decemlineata.
The newly-ecdysed third-instar larvae have ingested potato leaves immersed with water (control), 0.1 μg/mL Pyr or JH for 1 day (A). Otherwise, the newly-ecdysed third-instar larvae have ingested PBS-, dsegfp-, or dsAS-C-dipped leaves (B–D); or PBS-, dsegfp-, or dsJHAMT-dipped leaves (E–G) for 3 days. Different letters indicate significant difference at P value < 0.05.