Table 2 The contents of seven amino acids in the bodies and feces of the Leptinotarsa decemlineata larvae fed on PBS, dsegfp, dsNAT1-1 and dsNAT1-2-immersed foliage for 3 days.

From: Knockdown of a nutrient amino acid transporter gene LdNAT1 reduces free neutral amino acid contents and impairs Leptinotarsa decemlineata pupation

Amino acid

Origin

Concentration (μmol/g)

PBS

dsegfp

dsNAT1-1

dsNAT1-2

The third-instar larvae

 C

Bodies

11.12 ± 0.51 a

13.12 ± 0.84 a

3.21 ± 0.21 b

2.67 ± 0.14 b

   Feces

0.25 ± 0.02 b

0.33 ± 0.03 b

2.34 ± 0.14 a

3.15 ± 0.13 a

 H

Bodies

20.88 ± 1.24 a

20.02 ± 1.11 a

10.70 ± 0.52 b

11.17 ± 0.47 b

   Feces

4.69 ± 0.20 b

4.06 ± 0.28 b

8.84 ± 0.41 a

7.87 ± 0.52 a

 I

Bodies

41.49 ± 2.22 a

42.16 ± 3.04 a

26.77 ± 1.29 b

28.13 ± 1.38 b

   Feces

15.41 ± 0.84 b

12.68 ± 0.61 b

27.22 ± 1.15 a

29.39 ± 1.74 a

 L

Bodies

58.48 ± 2.41 a

58.76 ± 1.98 a

32.45 ± 2.07 b

34.68 ± 2.15 b

   Feces

20.31 ± 1.06 b

19.75 ± 1.19 b

37.55 ± 1.24 a

33.17 ± 2.03 a

 M

Bodies

1.74 ± 0.38 a

4.26 ± 0.25 a

0.64 ± 0.04 b

0.13 ± 0.01 b

   Feces

0.02 ± 0.00 b

0.03 ± 0.00 b

0.31 ± 0.02 a

0.25 ± 0.02 a

 F

Bodies

27.55 ± 1.07 a

28.64 ± 1.13 a

19.20 ± 1.23 b

15.73 ± 1.19 b

   Feces

11.56 ± 0.81 b

9.62 ± 0.57 b

23.69 ± 1.04 a

18.96 ± 1.07 a

 S

Bodies

55.50 ± 2.11 a

55.86 ± 4.08 a

41.88 ± 2.58 b

34.26 ± 2.14 b

   Feces

13.27 ± 1.01 b

11.67 ± 0.72 b

31.84 ± 1.22 a

29.62 ± 1.57 a

The fourth-instar larvae

 C

Bodies

6.89 ± 0.51 a

10.50 ± 0.67 a

2.10 ± 0.16 b

0.25 ± 0.02 c

   Feces

0.25 ± 0.02 b

0.25 ± 0.02 b

4.58 ± 0.37 a

7.70 ± 0.51 a

 H

Bodies

21.32 ± 0.71 a

19.86 ± 0.54 a

9.44 ± 0.35 b

10.50 ± 0.49 b

   Feces

4.69 ± 0.57 b

4.66 ± 0.41 b

8.64 ± 0.37 a

12.06 ± 0.61 a

 I

Bodies

39.21 ± 1.13 a

45.54 ± 2.02 a

21.79 ± 1.51 b

20.38 ± 1.44 b

   Feces

15.40 ± 0.87 b

13.72 ± 0.74 b

30.73 ± 1.57 a

27.28 ± 1.63 a

 L

Bodies

65.00 ± 3.54 a

63.12 ± 4.13 a

38.40 ± 2.33 b

37.77 ± 1.79 b

   Feces

23.30 ± 1.47 b

26.91 ± 1.56 b

46.79 ± 3.64 a

48.52 ± 4.11 a

 M

Bodies

1.60 ± 0.11 a

1.54 ± 0.08 a

0.65 ± 0.02 b

0.41 ± 0.01 b

   Feces

0.15 ± 0.01 b

0.40 ± 0.02 b

2.64 ± 0.15 a

1.51 ± 0.11 a

 F

Bodies

26.25 ± 1.47 a

29.72 ± 1.55 a

11.51 ± 0.71 b

14.34 ± 0.57 b

   Feces

11.55 ± 0.57 b

12.97 ± 0.69 b

22.34 ± 1.21 a

24.58 ± 1.54 a

 S

Bodies

52.88 ± 3.74 a

59.52 ± 3.69 a

26.64 ± 1.27 b

28.63 ± 1.34 b

   Feces

16.27 ± 0.79 b

15.34 ± 0.85 b

31.11 ± 1.45 a

42.19 ± 1.85 a

  1. Free amino acid contents in the bodies and feces are analyzed with a Beckman 6300 Amino Acid Analyzer. Difference of amino acid content within each line is analyzed by ANOVA followed by the Tukey-Kramer test. Data that do not share the same letters are significantly different at P values of 0.05.
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