Figure 3
From: Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity
hPSC culture on PVA-oligoVN hydrogels with different surface densities of oligoVN.
(A) Morphology of hESCs (WA09) cultured on PVA-oligoVN hydrogels with several surface densities of oligoVN (PVA-24h-50, PVA-24h-100, PVA-24h-250, PVA-24h-500, PVA-24h-1000 and PVA-24h-1500), Synthemax II dishes and Matrigel at passage 1. The red arrows indicate detached cells. The bar indicates 100 μm. (B) Attachment ratio of hPSCs (blue bar, hESCs [WA09] and red bar, hiPSCs [HPS0077]) on PVA-oligoVN hydrogels with several surface densities of oligoVN, Synthemax II dishes and Matrigel at passage 3. (D) Differentiation ratio of hPSCs (blue bar, hESCs [WA09] and red bar, hiPSCs [HPS0077]) on PVA-oligoVN hydrogels with several surface densities of oligoVN, Synthemax II dishes and Matrigel at passage 3.
