Figure 1 | Scientific Reports

Figure 1

From: In vivo cellular imaging of various stress/response pathways using AAV following axonal injury in mice

Figure 1

Construction and in vitro assessment of 7 pathway reporters (a) Schematic representation of the reporter vectors. Each stress/response promoter element was designed to drive EGFP reporter gene expression.

MiniP, minimal promoter, which contains the TATA-box, is required for stable transcription. The six response elements do not contain the TATA-box within the sequence; thus, miniP was fused to each of the elements. Meanwhile, the Mcp-1 promoter did not require miniP as it has its own internal TATA box; hGh pA, human growth hormone polyA signal. (b) Results of the in vitro reporter assay using specific inducers. Fluorescence relative to baseline values measured in cells with no pathway inducer added is presented. Positive dose-response relations were observed for all constructs. The bars represent means ± S.E.M. (N = 6 each).

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