Figure 2
In vivo imaging of RGC apoptosis in murine ONC.
(a) Representative images of apoptotic RGCs stained with Annexin V-FITC before and 1, 3, 5 and 7 days after ONC obtained using in vivo confocal microscopy. The upper panel shows images from the control eye, whereas the lower panel shows image from the ONC eyes. Note that Annexin V labelling and imaging were conducted on a different set of mice for each time point (N = 6 each) (b) Retinal section showing co-localization of Annexin V and cleaved caspase 3 in the RGC layer following ONC. Annexin V (green); cleaved caspase 3 (red). (c) Quantification of fluorescence-positive RGCs from images acquired through in vivo imaging of dying cells. The peak in RGC death occurred at day 5. Images were only a single picture with the optic nerve in the centre. Data represent means ± S.E.M. **P < 0.05, *P < 0.1. Scale bar: 50 μm. ONC, optic nerve crush injury; No Tx, no treatment.
