Figure 3
In vivo imaging of stress/response pathways in RGCs using AAV reporters.
(a) Representative images of stress responses in RGCs before and 1, 3, 5 and 7 days after ONC obtained using in vivo confocal microscopy. Upper panels display the images from the control eye whereas the lower panels display images from the ONC eyes. (b) Magnified image of the inset indicated in (a). (c) Quantification of EGFP-positive RGCs from images acquired through in vivo imaging (Mcp-1 and NF-κB, N = 5 each; TGF-β, N = 6; ARE, ATF6 and p53, N = 7 each; HRE, N = 8). Data were acquired from AAV2/2 reporter-injected mice with and without ONC. For each eye, a single picture with the optic nerved in the centre was analyzed. Increased EGFP+ cells (%) was determined by dividing the increase in the number of EGFP-positive cells from baseline by the number of cells observed at the baseline. Data represent means ± S.E.M. *P < 0.05. ONC, optic nerve crush injury; No Tx, no treatment. ARE, antioxidant response element; ATF6, ATF6 response element; HRE, hypoxia response element; Mcp-1, Mcp-1 promoter; NF-κB, NF-κB response element; p53, p53 response element; TGF-β, SMAD-binding element.
