Figure 1
NDM29 induces the down-regulation of ABC transporters in NB cells and its expression is pharmacologically induced by perhexiline maleate.
Expression levels of ABCA1 ((a) p < 0.0001), ABCA12 ((b) p < 0.0001) and SLC7A11 ((c) p = 0.0113) in Mock and S1 cells, measured by Real-time RT-PCR. One star (*) indicates p < 0.05, while two stars (**) indicate p < 0.001, two-tailed Student’s test. Results of the primary screening for perhexiline maleate (d). Results are reported as luciferase emission of treated vs. untreated cells. Luminescent signal is normalized to amounts of GPF of cells, for normalization of transfection efficiency. Effect of perhexiline (0.01-0.1-1-25-50 μM) (e) on the viability of SH-SY5Y wt was measured based on the dose–response curves of the cell index by the xCELLigence system. Cell Index (CI) was recorded every 30 minutes. Each trace at each concentration was an average of three replicates. Vehicle (DMSO): red curve; Perhexiline 0.01 μM: green curve; Perhexiline 0.1 μM: blue curve; Perhexiline 1 μM: pink curve; Perhexiline 25 μM: cyan curve; Perhexiline 50 μM: violet curve.
