Figure 2

Perhexiline treatment increases the susceptibility of NB cells to antiblastic treatments.

Real-time RT-PCR detection NDM29 mRNA amount in SH-SY5Y wt cells treated or untreated with perhexiline (0.01 and 1 μM) (24-48-72 h) (a). Values are mean ± SD. One star (*) indicates p < 0.05, while two stars (**) indicate p < 0.001, two-tailed Student’s test. Expression levels of ABCA1 ((b) p = 0.004), ABCA12 ((c) p = 0.0015) and SLC7A11 ((d) p = 0.0015) in SH-SY5Y wt cells treated with perhexiline (0.01 μM), measured by Real-time RT-PCR. One star (*) indicates p < 0.05, while two stars (**) indicate p < 0.001, two-tailed Student’s test. (e) Kinetics of cytotoxicity responses for cisplatin in SH-SY5Y wt cells, treated or not with perhexiline (0.01 μM), monitored by the RT-CES system. Cell Index (CI) was recorded every 30 minutes. Each trace at each concentration was an average of at least two replicates. Data are normalized to the time of compound addition of cell culture. (f) Capacity of SH-SY5Y wt to form colonies in methylcellulose in presence of different treatments (perhexiline 0.01 μM+ cisplatin 0.5 μM vs.cisplatin, p < 0.001). The data of two independent experiments are presented as the mean ± SD (n = 5 microscope fields for each treatment). The difference is significant as compared to cisplatin treatment: two stars (**) indicate p < 0.001, two-tailed Student’s test.