Figure 3
Perhexiline, in combination with chemotherapy, reduces growth of NB in vivo and increases survival.
Kaplan-Meier log rank test for survival, showing the effect of perhexiline on cisplatin-induced reduction of SK-N-BE(2) xenograft growth in the protocol a (vehicle, Perhexiline 1 mg/Kg/dose and/or Cisplatin 3 mg/Kg/dose) (a) and in the protocol b (vehicle, Perhexiline 3 mg/Kg/dose and/or Cisplatin 5 mg/Kg/dose) (b). Treatment started when the tumour diameter 0.5 mm in size. NOD-SCID mice were randomized to receive the following treatments: vehicle (DMSO) daily by oral gavage (pink curves), perhexiline daily by oral gavage (yellow curves), cisplatin weekly by intraperitoneal injection (blue curves), or the combination (Combo) of perhexiline and cisplatin daily (cyan curves). Tumour volumes were measured weekly. Mice were sacrificed when the tumor size reached 2 cm3 or greater. Effect of perhexiline on percentaged survival of NOD-SCID mice in protocol a (vehicle, Perhexiline 1 mg/Kg/dose and/or Cisplatin 3 mg/Kg/dose) (c) and protocol b (vehicle, Perhexiline 3 mg/Kg/dose and/or Cisplatin 5 mg/Kg/dose) (d). Vehicle (DMSO): pink curves, Perhexiline: yellow curves, Cisplatin: blue curves, or the combination (Combo) of Perhexiline and Cisplatin: cyan curves. Mice were sacrificed when the tumor size reached 2 cm3 or greater. The histograms reported the days on average needed to reach tumour volume threshold in protocol a (vehicle, Perhexiline 1 mg/Kg/dose and/or Cisplatin 3 mg/Kg/dose) (e) and protocol b (vehicle, Perhexiline 3 mg/Kg/dose and/or Cisplatin 5 mg/Kg/dose) (f). Values are mean ± SD. The star (*) indicates p < 0.05, two-tailed Student’s test.
