Figure 2
Silica NPs-induced ROS production and cell death in HEK293 cells with different TRPM2 expression levels.
(a,b) Summary of intracellular ROS production in HEK293 cells after exposed to the indicated concentrations of silica NPs for 3 h (a) and 6 h (b). Untreated cells were used as negative controls and cells treated with 1 mM H2O2 as positive controls. (c–f) Summary of the relative viability of HEK293 cells after exposed to the indicated concentrations of silica NPs for 3 h (c) and 6 h (d) alone, or together with NAC (10 mM; (c,d)) or SA (5 mM; (e,f )). Untreated cells were used as negative controls. The data are from 8 independent experiments, *P < 0.05, **P < 0.01, ***P < 0.005.
