Figure 1 | Scientific Reports

Figure 1

From: NMR structure and function of Helicoverpa armigera sterol carrier protein-2, an important insecticidal target from the cotton bollworm

Figure 1

The expression of recombinant GST-HaSCP-2 in E. coli and western blotting analysis. In the experiment of HaSCP-2 expression, the supernatant of E. coli lysate was used for SDS-PAGE and western blotting analysis. Protein samples were loaded on 15% SDS-PAGE. Lane 1: molecular weight standards (kDa); Lane 2: the total protein of E. coli BL21(DE3) carrying plasmid pGEX-KG with IPTG induction, *indicated the GST protein with a molecular weight of 26.5 kDa; Lane 3: the total protein of E. coli BL21 (DE3) carrying plasmid of pGEX-KG-HaSCP-2 without IPTG induction; Lane 4: total protein of E. coli BL21(DE3) carrying plasmid of pGEX-KG-HaSCP-2 with IPTG induction, **indicated the GST-HaSCP-2 fusion protein with a molecular weight of about 42 kDa; Line5: purified HaSCP-2 protein with molecular weight of about 16 kDa; Line6: purified truncated HaSCP-2 (trHaSCP-2) protein with molecular weight of about 14 kDa; For western blotting detection with anti-AeSCP-2 antibodies (anti-Aedes aegypti antibodies), Lane 7: the same molecular weight standards as Lane 1; Lane 8: purified HaSCP-2 protein was dected; Lane 9: purified truncated trHaSCP-2 protein was detected.

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