Figure 6 | Scientific Reports

Figure 6

From: NMR structure and function of Helicoverpa armigera sterol carrier protein-2, an important insecticidal target from the cotton bollworm

Figure 6

NBD-cholesterol binding assay of HaSCP-2.

Both full length protein and truncated protein (trHaSCP-2) were used for cholesterol binding assay. The net changes in NBD-cholesterol fluorescence in intensity (RFU = Relative fluorescence unit) was shown. The protein concentration was from 0.025 μM to15 μM and the final concentration of NBD-cholesterol for each assay was 0.25 μM. GST was used as the negative control. The dates were processed using GraphPad PRISM4.0 software.

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