Figure 7
The NBD-cholesterol binding assay of wild type and mutant HaSCP-2s.
The net changes in NBD-cholesterol fluorescence in intensity (RFU = Relative fluorescence unit) was showed with the concentration of NBD-cholesterol was 0.25 μM and in the presence of increasing concentrations of each protein (0.025 to 15 μM). The background NBD-cholesterol fluorescence was deducted from each assay. The data from three replicates for each assay was processed using GraphPad PRISM4.0 (single binding site and nonlinear regression model). R2 values of each non-linear regression curve were shown.
