Table 5 Difference between Wild Type HaSCP-2 and Mutants in NBD-Cholesterol binding.

From: NMR structure and function of Helicoverpa armigera sterol carrier protein-2, an important insecticidal target from the cotton bollworm

 

WT

Y51A

Y51W

F53A

F53W

F89A

F110W

I117M

Q131A

Kda (μM)

2.78

2.65

1.00

3.07

3.41

3.27

3.24

1.80

3.77

P valueb

−

0.023

0.09

0.031

0.026

0.038

0.019

0.043

0.034

  1. aKd values were determined using the GraphPad Prism version 4.0 (single-binding site, nonlinear regression model).
  2. bP values vs. wild type were given for Dunnett’s post T test between the wild type HaSCP-2 and each mutant after a repeated measures analysis of variance.
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