Figure 5

Hoechst 33258 staining assay of HepG2 apoptosis to the stimulations of Akbu-LAAO and exogenous H2O2.
HepG2 cells were incubated in the presence or absence of catalase for 1 h, treated with Akbu-LAAO or exogenous H2O2 for 24 h and stained Hoechst 33258. Nuclear condensation and/or fragmentation represent cell apoptosis. Images were taken at a magnification of 200×. Arrows marked the apoptotic cells for representing nuclear condensation/fragmentation.