Figure 2
From: Predicting the impact of promoter variability on regulatory outputs
Variability of the BqsR operator’s repeated pentamer sequence and its influence on gene regulation.
(A) The reference operator found in the PA14_04180 promoter region contains two pentamers separated by a 6-bp spacer region. (B) T he graph shows the PWM score and sequence logo for the upstream and downstream pentamer sequences calculated using the operators from Fig. 1. (C) Expression measurements of synthetic constructs quantified the influence of each point mutation for all nucleotides in the upstream pentamer on gene expression. Error bars show standard error of biological triplicates.
