Figure 3
From: Predicting the impact of promoter variability on regulatory outputs
Variability of the spacer sequence and its influence on gene regulation.
(A) The number of operators containing spacers of length 5, 6, or 7 bp is plotted for potential operators containing either 0 or 1 mutations in the pentamer regions, revealing a preference for spacer regions of length 6 bp. (B) Experimental measurements of synthetic operators confirm that a 6-bp spacer length is critical for BqsR-mediated regulation, as spacer lengths of 5 or 7 bp resulted in expression levels similar to the negative control in which the upstream pentamer was deleted. (C) Operators from (A) containing a 6-bp spacer region were analyzed for sequence preference by calculating the PWM score and sequence logo. (D) Gene expression measurements of 6 synthetic spacer sequences showed that the sequence of the spacer region modulates the level of gene regulation up to a factor of 2.
