Figure 4
From: Predicting the impact of promoter variability on regulatory outputs
Promoter regions with multiple operators.
(A) The number of operators within each promoter region of the genome as compared to randomly distributing the same number of operators throughout the genome. Operators are sequences 16 bp in length and contain up to 2 mutations in the pentamer regions. (B) Several of these clustered promoters are spaced by 6 bp, the same spacing between the two pentamer regions of an individual promoter, creating arrays of overlapping binding sites as shown in the schematic. (C) The promoter for PA14_04180 is one such promoter containing 4 repeated pentamer regions. (D) Gene expression measurements on synthetic versions of the PA14_04180 promoter, in which individual or pairs of pentamer repeats were removed, revealed that each pentamer repeat contributes to the overall level of gene regulation, although not all the BqsR binding sites contribute equally to the regulatory output.
