Figure 5
From: Predicting the impact of promoter variability on regulatory outputs
Creating a binding energy matrix for the BqsR operator sequence.
(A) The reference operator sequence from gene PA14_04180. (B) Energy matrix derived from the experimental measurements of regulation by synthetic operators. (C) For comparison, the energy matrix derived from the position weight matrix used for prediction shown in Fig. 1B, which was constructed using qPCR expression data for 12 genes. Binding energies in kBT units are relative to the binding strength to the reference operator in (A). Matrix positions in dark red indicate bp changes that cause large reductions in expression levels, whereas matrix positions in dark blue indicate bp that increase expression levels.
