Figure 8

Fold change in expression due to ferrous iron shock for promoters coregulated by both BqsR and RhlR.

For genes containing both BqsR and RhlR binding (bqsP, PA14_01240, PA14_04180 and PA14_07000), the fold change in expression due to Fe(II) shock was decreased upon overexpression of RhlR, demonstrating the ability of RhlR to modulate the effect BqsR has on expression at these promoters. Expression from the oprH promoter, which does not contain an RhlR binding site, was not significantly influenced by RhlR overexpression. The inset shows the bqs promoter, which contains overlapping operators. WT response is shown in white, ∆bqsR is shown in red and WT overexpressing rhlR is shown in blue. *indicates a p-value < 0.05.