Figure 4

Repair of sarcolemmal injury is not affected by Annexin A1 deficiency.

(A) Time-lapse images of myofibers of freshly isolated w.t. or Anx A1−/− biceps muscle before as well as 25 and 125 seconds after laser injury (white arrow indicates site of injury). Entry of dye into the myofiber is shown by the overlay of FM dye fluorescence (green) on the bright field (grayscale) image of the myofibers. (B) Quantification of FM1-43 influx, following laser injury into fibers isolated from w.t. in presence or absence of calcium (n = 19 fibers in presence and n = 10 in absence) and Anx A1−/− in presence of calcium (n = 20 fibers) from 2 mice for each condition. The plots show FM dye intensity averaged for all the fibers at indicated times following injury. The value at each time point is normalized to the dye intensity for the fiber at the time of injury (F0) and the error bars represent SEM. Scale bar 10 μm. Statistical difference between the kinetics of increase in averaged FM dye intensity were compared by two-tailed unpaired t-test and statistical significance of the difference in kinectis is shown as *** for p ≤ 0.001 compared to w.t. and ^### for p < 0.001 compared to Anx A1−/−.