Figure 3

(A) Time-dependent study of the ERE-containing AuNP dimers interaction with 10 nM ERβ over 30 minutes. The complex peak was observed at 5 minutes and the decrease in dimer peak intensity was accompanied by the growth of the complex peak (B) The dimers were queried with different concentrations of ERβ (1, 2.5, 5 and 10 nM) at 30 minutes after ERβ addition. The intensity of the complex peak relative to the dimer peak was incrementally higher as more ERβ was used.