Figure 5
DHT promoted PTEN expression by enhancing PPARγ expression in ovarian granulosa cells.
(A) Rat granulosa cells were pretreated with siRNAs of p53, Egr-1 and PPARγ for 24 hours, then the protein levels of p53, Egr-1, PPARγ were determined by western blot and significantly decreased after corresponding siRNAs treatment. *P < 0.05. (B) Rat granulosa cells were pretreated with siRNAs of p53, Egr-1 and PPARγ respecitvely for 24 hours prior DHT (25uM) treatment for another 24 hours, then PTEN mRNA expression was determined by Q-RT-PCR. PPARγ siRNA significantly suppressed the DHT-upregulated PTEN expression in ovarian granulosa cells. *P < 0.05. (C) PPARγ protein levels were determined by western blot after treatment of different doses of DHT for 24 hours in rat granulosa cells. The levels of PPARγ were significantly increased with incremental dosage of DHT treatment. Statistical comparisons were done between lane 1 with the other lane, *P < 0.05, N = 3. (D) PPARγ mRNA levels were determine by Q-RT-PCR after treatment of different doses of DHT for 24 hours in rat granulosa cells. The mRNA levels of PPARγ were significantly increased with incremental dosage of DHT treatment in rat granulosa cells, but decreased with bicalutamide treatment. Between-group comparisons were performed as indicated *P < 0.05, N = 3.
