Figure 6 | Scientific Reports

Figure 6

From: The effect of androgens on ovarian follicle maturation: Dihydrotestosterone suppress FSH-stimulated granulosa cell proliferation by upregulating PPARγ-dependent PTEN expression.

Figure 6

Expression of PCNA, p-Akt, PTEN, PPARγ and apoptosis marker in large antral follicles of placebo-, DHEA- and DHT-treated rats.

(A) The expression of PCNA, p-Akt, PTEN, PPARγ and TUNEL assay. The representative immunohistochemical staining image of PCNA, p-Akt, PTEN, PPARγ and the TUNEL assay results using ovaries from placebo-, DHEA- and DHT-treated rats are presented at 400x magnification. (B) Quantitative results of immunohistochemistry analysis. A total of 5 follicles per ovary were counted for analysis of granulosa cells with positive staining and 3 ovaries per group and 1 ovary per rat were collected for analysis. The data are shown as means ± SD and represent comparisons with the control group *P   < 0.05. (C) Western blot analysis of PTEN and PPAR-γ proteins in ovarian tissue (N = 3/group). Each ovarian tissue was homogenized and extracted, the equal amount of protein extracts were subjected to immunoblotting as described in the materials and methods. Actin was used as an internal control.

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