Figure 7
From: GFP-complementation assay to detect functional CPP and protein delivery into living cells
Recapitulation of CPP functional activity and ranking in an independent cell toxicity assay.
Synthetic peptides are added to T47D cells and incubated for 24 hours in a DPMI-α cell toxicity assay, where peptide uptake results in a decrease in cell viability. Dose-dependent peptide toxicity is only detected for CPP DPMI-α fusions at both 24 h (7a) and 48 h (7b), illustrating CPP-dependent internalization. Here, “No CPP” control is the DPMI-α peptide without a CPP. Data is representative of two independent experiments. Error bars represent standard error of the mean between technical replicates.
